The Bicolour Rubber Bone is an item that falls under the category of "Sports" in the Supplies. It is no different from the other three Rubber Bones besides colour. It is striped yellow and a pinkish red colour with black "Nintendogs" text engraved on it.
"This toy is great for strengthening a puppy's jaw and helping it relieve stress."
- Even though the Bicolour Rubber Bone claims to be made of rubber, when a dog grabs it, it sounds as though it is grabbing crystal.
- In addition to sounds, the bone makes a spring-like sound upon hitting something.